Chuck Person's Eccojams Vol. 1 is an album by American electronic musician Daniel Lopatin under the pseudonym Chuck Person. A plunderphonics and chopped and screwed album, Eccojams Vol. 1 features songs that consist of looped samples from popular songs from the 1980s and 1990s, with effects such as pitch shifting being applied. The album has an overall mournful tone and often takes melancholic lyrics from the songs it samples.
Eccojams Vol. 1 has been described as plunderphonics,[2] chopped and screwed,[19] and vaporwave.[20] The tone of the album has been described as "dystopian",[21] "unnerving, often mournful",[22] "vast and mysterious",[18] and "somber-yet-tropical".[23] The songs consist of looped samples of popular songs from the 1980s and 1990s with added effects such as pitch shifting and reverb,[13][22] a technique derived DJ Screw's chopped and screwed technique[13] and likened to a "candy-coloured variation" of William Basinski's The Disintegration Loops.[24] Most of the lyrics isolated in these loops are lyrics that differ from "overall tone and sentiment" of the original songs and often express negative feelings. An example would be "B4", which isolates the lyric "There's nobody here" from "The Lady in Red" to convey existentialism, which differs from the romantic tone of the original song.[13]
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"B1" has a bleak tone, similar to a twisted dream, before transitioning to an uplifting loop of Kate Bush's voice from "Don't Give Up".[1][25] "B2" is a "drunken, off-key" and slowed-down loop of "Gypsy". "B5" samples 2Pac's "Me Against the World".[18] The final song, "B7", is a loop of "Woman in Chains" by Tears for Fears that has shimmering effect "approaching and receding" that eventually "overtakes the words" before the song fades out.[1]
Though Eccojams Vol. 1 was released "with little fanfare",[24] the videos that Lopatin posted on YouTube became relatively popular, with "Nobody Here" amassing 30 thousand views over several months. Music critic Simon Reynolds highlighted the videos' "conceptual framework" as "relat[ing] to cultural memory and the buried utopianism within capitalist commodities, especially those related to consumer technology in the computing and audio/video entertainment area".[5] Anthony Fantano mentioned Eccojams Vol. 1 in his 2012 review of Macintosh Plus's Floral Shoppe as an album that he found to be "more bold with its editing and its looping and its stretching" of music samples.[26]
Samples usually come in a WAV or MP3 format, which can be imported into your favorite music production software to make music.We hope you found this ultimate list of free drum kits and drum samples helpful.What are your favorite free drum kits? Let us know in the comments.
The QIAGEN Plasmid Kits uses gravity-flow QIAGEN anion-exchange tips for efficient purification of plasmid DNA. Up to 10 mg (giga), 2.5 mg (mega), 500 µg (maxi), 100 µg (midi), and 20 µg (mini) high-copy plasmid DNA is purified from culture (culture volumes depend on plasmid copy number, size of insert, host strain, and culture medium). Plasmid DNA purified with QIAGEN Plasmid Kits is highly suitable for use in applications such as transfection (see figure " Transfection efficiency versus plasmid purification method"), cloning, and in vitro transcription.
Running fractions saved from each step in the plasmid preparation procedure on an agarose gel enables monitoring the performance of each crucial step in the protocol. If the plasmid DNA is of low yield or quality, the samples can be analyzed to determine at what stage of the purification procedure the difficulty occurred.
For Research Use Only. Not for use in diagnostic procedures.FeaturesSpecificationsDownstream applicationCloning, NGS, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern BlottingElution volume15 µL or aboveStarting materialDNA or RNA: PCR products, gDNA, cDNAStarting amountScalableDNA recovered>90% recovery for DNA >100 bpProcessing modeAutomated; manualThroughput96-384 samples per runDNA binding technologyMagnetic beadsStorage2C - 8CSpecial noteSize selection by varying beads ratioProtocol and ResourcesProduct Documentation & Literature
Our IMAC PhyTip columns are packed with high capacity Nickel-IMAC affinity resin and are used to purify biologically functional, recombinant His-tagged recombinant proteins and consistently generate purified samples of high purity and concentration by dual flow chromatography.
PhyNexus now part of Biotage developed the PhyTip column in 2002 and have supported researchers with automated protein purification ever since. PhyTip columns purified samples by repeated back-and-forth cycles - a process called dual flow chromatography - allowing complete binding interactions in each purification step.
The dissemination of particular MRSA lineages is correlated with their resistance profiles. The majority of the multiresistant type I MRSA isolates, predominant on both continents, lacked susceptibility to tetracycline, erythromycin, clindamycin, gentamicin, ciprofloxacin, and rifampin. A second multiresistant lineage (type IIb), found in North America and several European countries, was susceptible to tetracycline and rifampin. Smaller pandemics were caused by isolates susceptible to gentamicin, tetracycline, and rifampin (type IIa and type IIIb). Although antibiotic selection pressure by itself provides a reasonable explanation for the widespread dissemination of such multiresistant strains, additional factors, e.g., modifications in expression of virulence factors and binding capacities, may add to their high prevalence. In contrast, the sporadically isolated mecA+ MRSA types, type IIIa and types IV to VIII (1% of recent European isolates), generally remained susceptible to all except the β-lactam antibiotics. Sporadic MRSA types were overrepresented in the samples from the community in Chicago (20%) and UMC (30%), where the search-and-destroy procedure prevents epidemic MRSA strains from entering the hospital, keeping the prevalence of MRSA isolates below 1%. In both the community and the hospital, sporadic MRSA types, which are not descendants of the epidemic hospital clones, may be formed de novo by the horizontal transfer of mecA to resident MSSA lineages, as was witnessed recently in vivo when the gene was passed from S. epidermidis to S. aureus during antibiotic treatment (21).
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In order to build the city and support all the procedurally generated instances that make up the world, Nanite needed to be used as much as possible. It meant that a mega mesh could be built and needed to use instances to do so. Each building in the city is made up of hundreds of instances, and all props on the street, such as furniture, decals, and even trash are using Nanite. Very little custom geometry is used in the city, which ensures that the project's memory usage stays within the set budget.
You receive this error message because Outlook 2013 and later versions have a default attachment size limit of 20 megabytes (20480 KB) for Internet email accounts. This limit prevents your computer from continually trying to upload very large attachments that exceed the limits of most Internet service providers. This limit applies whether you are adding one large attachment that is greater than 20 megabytes (MB) or several attachments whose sum total size is greater than 20 MB.
The file format is basic INI:Sections of text, led by a [section] header and followed bykey=value entries on separate lines. In rclone each remote isrepresented by its own section, where the section name defines thename of the remote. Options are specified as the key=value entries,where the key is the option name without the --backend- prefix,in lowercase and with _ instead of -. E.g. option --mega-hard-deletecorresponds to key hard_delete. Only backend options can be specified.A special, and required, key type identifies the storage system,where the value is the internal lowercase name as returned by commandrclone help backends. Comments are indicated by ; or # at thebeginning of a line. 2ff7e9595c
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